Coffee is known to increase pancreatic secretion of digestive enzymes. The mutagen, aflatoxin B1(AFB1) is contained in fermented foods and known to increase the specific activities of pancreatic chymotrypsin, trypsin, amylase, and lipase.
Nowadays,
coffee intake is increased among Koreans who have consumed relatively high amount of traditional fermented foods. Therefore, this study was performed to examine the effect of coffee and AFB1 on pancreatic exocrine function and structure.
Rats were divided into 10 experimental, groups. the first five groups were W(control group). LD(0.2g decaffeinated coffee/Kg B.W), HD(3g decaffeinated coffee/Kg B.W), LC(0.2g coffe/Kg B.W), and HC(3g coffee/Kg B.W). The second five groups were
WA,
LDA,
HDA, LCA, HCA, same as first five groups in caffeine level but treated with AFB1.
The result of this experiment showed that the caffeine intake did not influence significantly on the growth and feed efficiency. But water intake was increased by caffeine intake and AFB1, treatment. the weights of pancreas and liver were
increased
as
the caffeine intake was increased. Trypsin activities were tend to increase in concentrated coffee groups(HD, HC), AFB1 treated groups showed the higher trypsin level than the AFB1 untreated groups, Amylase activities were tend to increase in
concentrated coffee groups(HD, HC) of AFB1 untreated animals. AFB1 treatment did not show the additional effect on the stimulated amylase secretion by coffee. Lipase activities were tend to decrease in concentrated coffee groups(HD, HC) of AFB1
untreated animals. Lipase activities were increased in the order named WA group, coffee groups. decaffeinated coffee groups in AFN1 treated animals. AFB1 treated groups showed the higher lipase level than AFB1 untreated groups. In the histologic
observation of pancreas HCA group showed more dense compound tubuloalveolar glands and proliferation of nuclei than normal. The results suggested a development of a atypia which is ongoing phase to a cancer .
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